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tmt10plex isobaric label reagent set  (Thermo Fisher)


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    Thermo Fisher tmt10plex isobaric label reagent set
    Tmt10plex Isobaric Label Reagent Set, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tmt10plex+isobaric+label+reagent+set/tmt10plex+isobaric+label+reagent+set/pmc12185105-100-5-13
    Average 90 stars, based on 1 article reviews
    tmt10plex isobaric label reagent set - by Bioz Stars, 2026-10
    90/100 stars

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    Recombinant:

    Article Title: Spatiotemporally resolved GPCR interactome uncovers unique mediators of receptor agonism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-FLAG M1 primary Sigma-Aldrich Cat# F3040, RRID: AB_439712 Goat anti-mouse Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11001, RRID: AB_2534069 Rabbit anti-EEA1 primary Invitrogen Thermo Fisher Scientific Cat# MA5-14794, RRID: AB_10985824 Goat anti-rabbit Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11008, RRID: AB_143165 Goat anti-mouse Alexa Fluor 594-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11005, RRID: AB_2534073 Chemicals, peptides, and recombinant proteins Luteinizing hormone (LH) GoldenWest BioSolutions, LLC Cat# TLIH-A1165.02 Lipofectamine 2000 Transfection Reagent Invitrogen Thermo Fisher Scientific Cat# 12566014 Zeocin Invitrogen Thermo Fisher Scientific Cat# R25001 Blasticidin Thermo Scientific Thermo Fisher Scientific Cat# R21001 Hygromycin B Invitrogen Thermo Fisher Scientific Cat# 10687010 Tetracycline hydrochloride Merck Cat# T7660-5G Pierce NeutrAvidinTM Agarose Beads Thermo Scientific Thermo Fisher Scientific Cat# 29201 Hydrogen peroxide solution 30% (w/w) in H2O, contains stabilizer Merck Cat# H1009-5ML Pierce Quantitative assay Thermo Scientific Thermo Fisher Scientific Cat# 23275 TMT10plex Isobaric Label Reagent Set Thermo Scientific Thermo Fisher Scientific Cat# 90110 Sequencing Grade Modified Trypsin, Mass Spec Grade Promega Promega Cat# V5111 Lys-C, Mass Spec Grade Promega Promega Cat# VA1170 Pierce High pH Reversed-Phased Kit Thermo Scientific Thermo Fisher Scientific Cat# 84868 Biotinyl Tyramide (Biotin Phenol) Tocris Bioscience Bio-Techne Cat# 6241 Pierce High Sensitivity NeutrAvidin-HRP Conjugate Thermo Fisher Scientific Thermo Fisher Scientific Cat# 31030 Luminata Western HRP Chemiluminescence Substrate Merck Scientific Laboratory Supplies Cat# WBLUC0100 GloSensor cAMP Reagent stock solution Promega Promega Cat# E1290 DharmaFECT 1 Transfection reagent Dharmacon Reagents Horizon Discovery Cat# T-2001-01 Critical commercial assays LANCE Ultra cAMP assay Perkin Elmer Revvity Cat# TRF0263 HTRF-based cAMP Gαs dynamic kit Revvity Revvity Cat# 62a.m.4PEC Deposited data Source data for mass spectrometry analyses This study PRIDE: PXD053246 Experimental models: Cell lines Flp-In T-REx 293 Francis Crick Institute Cell Facility, UK RRID:CVCL_U427 FLAG-LHR WT HEK293 Hanyaloglu lab; JeanAlphonse et al.22 N/A Flp-In T-REx 293 LHR-APEX2 cells This study N/A (Continued on next page) e1 Cell Chemical Biology 32, 722–735.e1–e7, May 15, 2025 ..

    Article Title: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich Cat#: F1804-1MG; RRID:AB_262044 His-Tag (27E8) Mouse mAb Cell Signaling Cat#: 2366; RRID:AB_2115719 Anti-NanoLuc Monoclonal Antibody Promega Cat#: N7000; RRID: AB_3095534 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody VWR Cat#: 926-32210; RRID:AB_621842 LC3B (D11) XP Rabbit mAb Cell Signaling Cat#: 3868; RRID: AB_2137707 Vinculin (E1E9V) XP Cell Signaling Cat#: 13901; RRID: AB_2728768 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#: C2987H Chemicals, peptides, and recombinant proteins Tetramethylrhodamine (TAMRA)-PEG3-azide Synthesized in-house N/A Biotin-PEG4-azide BroadPharm Cat#: BP-22119 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#: 32106 Novex 4-20% Tris-Glycine Mini Gels Invitrogen Cat#: XP04205BOX Nitrocellulose western blotting membrane, 0.45 mM GE Healthcare Amersham Cat#: 10600002 DMSO Corning Cat#: 25-950-CQC Urea Fisher Scientific Cat#: M1084871000 Iodoacetamide Sigma-Aldrich Cat#: I1149-25G Tris(benzyltriazolylmethyl)amine (TBTA) TCI Cat#: T2993 Copper(II) sulfate, anhydrous Sigma-Aldrich Cat#: 451657-10G Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#: 75259 Sequencing grade modified trypsin Promega Cat#: V5111 Streptavidin agarose resin Fisher Scientific Cat#: 20349 Micro bio-spin column Bio-rad Cat#: 7326204 Triethylammonium bicarbonate (TEAB) buffer Sigma-Aldrich Cat#: T7408-500ML TMT10plex Isobaric Label Reagent Set Thermo Scientific Cat#: 90406 TMT16plex Isobaric Label Reagent Set Thermo Scientific Cat#: 467804-10ML Formic acid, 98%, for mass spectrometry Honeywell Fluka Cat#: 94318-250ML-F Lipofectamine 3000 Transfection Reagent Thermo Scientific Cat#: L3000001 Polybrene Sigma Aldrich Cat#: TR-1003-G puromycin InvivoGen Cat#: ant-pr-1 PI-103 Sigma Aldrich Cat#: 528101 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#: G7573 NanoBRET Nano-Glo Substrate Promega Cat#: N1571 (Continued on next page) Cell Chemical Biology 32, 1–18.e1–e32, March 20, 2025 e1 ..

    Transfection:

    Article Title: Spatiotemporally resolved GPCR interactome uncovers unique mediators of receptor agonism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-FLAG M1 primary Sigma-Aldrich Cat# F3040, RRID: AB_439712 Goat anti-mouse Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11001, RRID: AB_2534069 Rabbit anti-EEA1 primary Invitrogen Thermo Fisher Scientific Cat# MA5-14794, RRID: AB_10985824 Goat anti-rabbit Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11008, RRID: AB_143165 Goat anti-mouse Alexa Fluor 594-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11005, RRID: AB_2534073 Chemicals, peptides, and recombinant proteins Luteinizing hormone (LH) GoldenWest BioSolutions, LLC Cat# TLIH-A1165.02 Lipofectamine 2000 Transfection Reagent Invitrogen Thermo Fisher Scientific Cat# 12566014 Zeocin Invitrogen Thermo Fisher Scientific Cat# R25001 Blasticidin Thermo Scientific Thermo Fisher Scientific Cat# R21001 Hygromycin B Invitrogen Thermo Fisher Scientific Cat# 10687010 Tetracycline hydrochloride Merck Cat# T7660-5G Pierce NeutrAvidinTM Agarose Beads Thermo Scientific Thermo Fisher Scientific Cat# 29201 Hydrogen peroxide solution 30% (w/w) in H2O, contains stabilizer Merck Cat# H1009-5ML Pierce Quantitative assay Thermo Scientific Thermo Fisher Scientific Cat# 23275 TMT10plex Isobaric Label Reagent Set Thermo Scientific Thermo Fisher Scientific Cat# 90110 Sequencing Grade Modified Trypsin, Mass Spec Grade Promega Promega Cat# V5111 Lys-C, Mass Spec Grade Promega Promega Cat# VA1170 Pierce High pH Reversed-Phased Kit Thermo Scientific Thermo Fisher Scientific Cat# 84868 Biotinyl Tyramide (Biotin Phenol) Tocris Bioscience Bio-Techne Cat# 6241 Pierce High Sensitivity NeutrAvidin-HRP Conjugate Thermo Fisher Scientific Thermo Fisher Scientific Cat# 31030 Luminata Western HRP Chemiluminescence Substrate Merck Scientific Laboratory Supplies Cat# WBLUC0100 GloSensor cAMP Reagent stock solution Promega Promega Cat# E1290 DharmaFECT 1 Transfection reagent Dharmacon Reagents Horizon Discovery Cat# T-2001-01 Critical commercial assays LANCE Ultra cAMP assay Perkin Elmer Revvity Cat# TRF0263 HTRF-based cAMP Gαs dynamic kit Revvity Revvity Cat# 62a.m.4PEC Deposited data Source data for mass spectrometry analyses This study PRIDE: PXD053246 Experimental models: Cell lines Flp-In T-REx 293 Francis Crick Institute Cell Facility, UK RRID:CVCL_U427 FLAG-LHR WT HEK293 Hanyaloglu lab; JeanAlphonse et al.22 N/A Flp-In T-REx 293 LHR-APEX2 cells This study N/A (Continued on next page) e1 Cell Chemical Biology 32, 722–735.e1–e7, May 15, 2025 ..

    Article Title: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich Cat#: F1804-1MG; RRID:AB_262044 His-Tag (27E8) Mouse mAb Cell Signaling Cat#: 2366; RRID:AB_2115719 Anti-NanoLuc Monoclonal Antibody Promega Cat#: N7000; RRID: AB_3095534 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody VWR Cat#: 926-32210; RRID:AB_621842 LC3B (D11) XP Rabbit mAb Cell Signaling Cat#: 3868; RRID: AB_2137707 Vinculin (E1E9V) XP Cell Signaling Cat#: 13901; RRID: AB_2728768 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#: C2987H Chemicals, peptides, and recombinant proteins Tetramethylrhodamine (TAMRA)-PEG3-azide Synthesized in-house N/A Biotin-PEG4-azide BroadPharm Cat#: BP-22119 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#: 32106 Novex 4-20% Tris-Glycine Mini Gels Invitrogen Cat#: XP04205BOX Nitrocellulose western blotting membrane, 0.45 mM GE Healthcare Amersham Cat#: 10600002 DMSO Corning Cat#: 25-950-CQC Urea Fisher Scientific Cat#: M1084871000 Iodoacetamide Sigma-Aldrich Cat#: I1149-25G Tris(benzyltriazolylmethyl)amine (TBTA) TCI Cat#: T2993 Copper(II) sulfate, anhydrous Sigma-Aldrich Cat#: 451657-10G Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#: 75259 Sequencing grade modified trypsin Promega Cat#: V5111 Streptavidin agarose resin Fisher Scientific Cat#: 20349 Micro bio-spin column Bio-rad Cat#: 7326204 Triethylammonium bicarbonate (TEAB) buffer Sigma-Aldrich Cat#: T7408-500ML TMT10plex Isobaric Label Reagent Set Thermo Scientific Cat#: 90406 TMT16plex Isobaric Label Reagent Set Thermo Scientific Cat#: 467804-10ML Formic acid, 98%, for mass spectrometry Honeywell Fluka Cat#: 94318-250ML-F Lipofectamine 3000 Transfection Reagent Thermo Scientific Cat#: L3000001 Polybrene Sigma Aldrich Cat#: TR-1003-G puromycin InvivoGen Cat#: ant-pr-1 PI-103 Sigma Aldrich Cat#: 528101 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#: G7573 NanoBRET Nano-Glo Substrate Promega Cat#: N1571 (Continued on next page) Cell Chemical Biology 32, 1–18.e1–e32, March 20, 2025 e1 ..

    Sequencing:

    Article Title: Spatiotemporally resolved GPCR interactome uncovers unique mediators of receptor agonism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-FLAG M1 primary Sigma-Aldrich Cat# F3040, RRID: AB_439712 Goat anti-mouse Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11001, RRID: AB_2534069 Rabbit anti-EEA1 primary Invitrogen Thermo Fisher Scientific Cat# MA5-14794, RRID: AB_10985824 Goat anti-rabbit Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11008, RRID: AB_143165 Goat anti-mouse Alexa Fluor 594-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11005, RRID: AB_2534073 Chemicals, peptides, and recombinant proteins Luteinizing hormone (LH) GoldenWest BioSolutions, LLC Cat# TLIH-A1165.02 Lipofectamine 2000 Transfection Reagent Invitrogen Thermo Fisher Scientific Cat# 12566014 Zeocin Invitrogen Thermo Fisher Scientific Cat# R25001 Blasticidin Thermo Scientific Thermo Fisher Scientific Cat# R21001 Hygromycin B Invitrogen Thermo Fisher Scientific Cat# 10687010 Tetracycline hydrochloride Merck Cat# T7660-5G Pierce NeutrAvidinTM Agarose Beads Thermo Scientific Thermo Fisher Scientific Cat# 29201 Hydrogen peroxide solution 30% (w/w) in H2O, contains stabilizer Merck Cat# H1009-5ML Pierce Quantitative assay Thermo Scientific Thermo Fisher Scientific Cat# 23275 TMT10plex Isobaric Label Reagent Set Thermo Scientific Thermo Fisher Scientific Cat# 90110 Sequencing Grade Modified Trypsin, Mass Spec Grade Promega Promega Cat# V5111 Lys-C, Mass Spec Grade Promega Promega Cat# VA1170 Pierce High pH Reversed-Phased Kit Thermo Scientific Thermo Fisher Scientific Cat# 84868 Biotinyl Tyramide (Biotin Phenol) Tocris Bioscience Bio-Techne Cat# 6241 Pierce High Sensitivity NeutrAvidin-HRP Conjugate Thermo Fisher Scientific Thermo Fisher Scientific Cat# 31030 Luminata Western HRP Chemiluminescence Substrate Merck Scientific Laboratory Supplies Cat# WBLUC0100 GloSensor cAMP Reagent stock solution Promega Promega Cat# E1290 DharmaFECT 1 Transfection reagent Dharmacon Reagents Horizon Discovery Cat# T-2001-01 Critical commercial assays LANCE Ultra cAMP assay Perkin Elmer Revvity Cat# TRF0263 HTRF-based cAMP Gαs dynamic kit Revvity Revvity Cat# 62a.m.4PEC Deposited data Source data for mass spectrometry analyses This study PRIDE: PXD053246 Experimental models: Cell lines Flp-In T-REx 293 Francis Crick Institute Cell Facility, UK RRID:CVCL_U427 FLAG-LHR WT HEK293 Hanyaloglu lab; JeanAlphonse et al.22 N/A Flp-In T-REx 293 LHR-APEX2 cells This study N/A (Continued on next page) e1 Cell Chemical Biology 32, 722–735.e1–e7, May 15, 2025 ..

    Article Title: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich Cat#: F1804-1MG; RRID:AB_262044 His-Tag (27E8) Mouse mAb Cell Signaling Cat#: 2366; RRID:AB_2115719 Anti-NanoLuc Monoclonal Antibody Promega Cat#: N7000; RRID: AB_3095534 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody VWR Cat#: 926-32210; RRID:AB_621842 LC3B (D11) XP Rabbit mAb Cell Signaling Cat#: 3868; RRID: AB_2137707 Vinculin (E1E9V) XP Cell Signaling Cat#: 13901; RRID: AB_2728768 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#: C2987H Chemicals, peptides, and recombinant proteins Tetramethylrhodamine (TAMRA)-PEG3-azide Synthesized in-house N/A Biotin-PEG4-azide BroadPharm Cat#: BP-22119 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#: 32106 Novex 4-20% Tris-Glycine Mini Gels Invitrogen Cat#: XP04205BOX Nitrocellulose western blotting membrane, 0.45 mM GE Healthcare Amersham Cat#: 10600002 DMSO Corning Cat#: 25-950-CQC Urea Fisher Scientific Cat#: M1084871000 Iodoacetamide Sigma-Aldrich Cat#: I1149-25G Tris(benzyltriazolylmethyl)amine (TBTA) TCI Cat#: T2993 Copper(II) sulfate, anhydrous Sigma-Aldrich Cat#: 451657-10G Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#: 75259 Sequencing grade modified trypsin Promega Cat#: V5111 Streptavidin agarose resin Fisher Scientific Cat#: 20349 Micro bio-spin column Bio-rad Cat#: 7326204 Triethylammonium bicarbonate (TEAB) buffer Sigma-Aldrich Cat#: T7408-500ML TMT10plex Isobaric Label Reagent Set Thermo Scientific Cat#: 90406 TMT16plex Isobaric Label Reagent Set Thermo Scientific Cat#: 467804-10ML Formic acid, 98%, for mass spectrometry Honeywell Fluka Cat#: 94318-250ML-F Lipofectamine 3000 Transfection Reagent Thermo Scientific Cat#: L3000001 Polybrene Sigma Aldrich Cat#: TR-1003-G puromycin InvivoGen Cat#: ant-pr-1 PI-103 Sigma Aldrich Cat#: 528101 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#: G7573 NanoBRET Nano-Glo Substrate Promega Cat#: N1571 (Continued on next page) Cell Chemical Biology 32, 1–18.e1–e32, March 20, 2025 e1 ..

    Modification:

    Article Title: Spatiotemporally resolved GPCR interactome uncovers unique mediators of receptor agonism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-FLAG M1 primary Sigma-Aldrich Cat# F3040, RRID: AB_439712 Goat anti-mouse Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11001, RRID: AB_2534069 Rabbit anti-EEA1 primary Invitrogen Thermo Fisher Scientific Cat# MA5-14794, RRID: AB_10985824 Goat anti-rabbit Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11008, RRID: AB_143165 Goat anti-mouse Alexa Fluor 594-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11005, RRID: AB_2534073 Chemicals, peptides, and recombinant proteins Luteinizing hormone (LH) GoldenWest BioSolutions, LLC Cat# TLIH-A1165.02 Lipofectamine 2000 Transfection Reagent Invitrogen Thermo Fisher Scientific Cat# 12566014 Zeocin Invitrogen Thermo Fisher Scientific Cat# R25001 Blasticidin Thermo Scientific Thermo Fisher Scientific Cat# R21001 Hygromycin B Invitrogen Thermo Fisher Scientific Cat# 10687010 Tetracycline hydrochloride Merck Cat# T7660-5G Pierce NeutrAvidinTM Agarose Beads Thermo Scientific Thermo Fisher Scientific Cat# 29201 Hydrogen peroxide solution 30% (w/w) in H2O, contains stabilizer Merck Cat# H1009-5ML Pierce Quantitative assay Thermo Scientific Thermo Fisher Scientific Cat# 23275 TMT10plex Isobaric Label Reagent Set Thermo Scientific Thermo Fisher Scientific Cat# 90110 Sequencing Grade Modified Trypsin, Mass Spec Grade Promega Promega Cat# V5111 Lys-C, Mass Spec Grade Promega Promega Cat# VA1170 Pierce High pH Reversed-Phased Kit Thermo Scientific Thermo Fisher Scientific Cat# 84868 Biotinyl Tyramide (Biotin Phenol) Tocris Bioscience Bio-Techne Cat# 6241 Pierce High Sensitivity NeutrAvidin-HRP Conjugate Thermo Fisher Scientific Thermo Fisher Scientific Cat# 31030 Luminata Western HRP Chemiluminescence Substrate Merck Scientific Laboratory Supplies Cat# WBLUC0100 GloSensor cAMP Reagent stock solution Promega Promega Cat# E1290 DharmaFECT 1 Transfection reagent Dharmacon Reagents Horizon Discovery Cat# T-2001-01 Critical commercial assays LANCE Ultra cAMP assay Perkin Elmer Revvity Cat# TRF0263 HTRF-based cAMP Gαs dynamic kit Revvity Revvity Cat# 62a.m.4PEC Deposited data Source data for mass spectrometry analyses This study PRIDE: PXD053246 Experimental models: Cell lines Flp-In T-REx 293 Francis Crick Institute Cell Facility, UK RRID:CVCL_U427 FLAG-LHR WT HEK293 Hanyaloglu lab; JeanAlphonse et al.22 N/A Flp-In T-REx 293 LHR-APEX2 cells This study N/A (Continued on next page) e1 Cell Chemical Biology 32, 722–735.e1–e7, May 15, 2025 ..

    Article Title: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich Cat#: F1804-1MG; RRID:AB_262044 His-Tag (27E8) Mouse mAb Cell Signaling Cat#: 2366; RRID:AB_2115719 Anti-NanoLuc Monoclonal Antibody Promega Cat#: N7000; RRID: AB_3095534 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody VWR Cat#: 926-32210; RRID:AB_621842 LC3B (D11) XP Rabbit mAb Cell Signaling Cat#: 3868; RRID: AB_2137707 Vinculin (E1E9V) XP Cell Signaling Cat#: 13901; RRID: AB_2728768 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#: C2987H Chemicals, peptides, and recombinant proteins Tetramethylrhodamine (TAMRA)-PEG3-azide Synthesized in-house N/A Biotin-PEG4-azide BroadPharm Cat#: BP-22119 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#: 32106 Novex 4-20% Tris-Glycine Mini Gels Invitrogen Cat#: XP04205BOX Nitrocellulose western blotting membrane, 0.45 mM GE Healthcare Amersham Cat#: 10600002 DMSO Corning Cat#: 25-950-CQC Urea Fisher Scientific Cat#: M1084871000 Iodoacetamide Sigma-Aldrich Cat#: I1149-25G Tris(benzyltriazolylmethyl)amine (TBTA) TCI Cat#: T2993 Copper(II) sulfate, anhydrous Sigma-Aldrich Cat#: 451657-10G Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#: 75259 Sequencing grade modified trypsin Promega Cat#: V5111 Streptavidin agarose resin Fisher Scientific Cat#: 20349 Micro bio-spin column Bio-rad Cat#: 7326204 Triethylammonium bicarbonate (TEAB) buffer Sigma-Aldrich Cat#: T7408-500ML TMT10plex Isobaric Label Reagent Set Thermo Scientific Cat#: 90406 TMT16plex Isobaric Label Reagent Set Thermo Scientific Cat#: 467804-10ML Formic acid, 98%, for mass spectrometry Honeywell Fluka Cat#: 94318-250ML-F Lipofectamine 3000 Transfection Reagent Thermo Scientific Cat#: L3000001 Polybrene Sigma Aldrich Cat#: TR-1003-G puromycin InvivoGen Cat#: ant-pr-1 PI-103 Sigma Aldrich Cat#: 528101 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#: G7573 NanoBRET Nano-Glo Substrate Promega Cat#: N1571 (Continued on next page) Cell Chemical Biology 32, 1–18.e1–e32, March 20, 2025 e1 ..

    Mass Spectrometry:

    Article Title: Spatiotemporally resolved GPCR interactome uncovers unique mediators of receptor agonism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-FLAG M1 primary Sigma-Aldrich Cat# F3040, RRID: AB_439712 Goat anti-mouse Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11001, RRID: AB_2534069 Rabbit anti-EEA1 primary Invitrogen Thermo Fisher Scientific Cat# MA5-14794, RRID: AB_10985824 Goat anti-rabbit Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11008, RRID: AB_143165 Goat anti-mouse Alexa Fluor 594-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11005, RRID: AB_2534073 Chemicals, peptides, and recombinant proteins Luteinizing hormone (LH) GoldenWest BioSolutions, LLC Cat# TLIH-A1165.02 Lipofectamine 2000 Transfection Reagent Invitrogen Thermo Fisher Scientific Cat# 12566014 Zeocin Invitrogen Thermo Fisher Scientific Cat# R25001 Blasticidin Thermo Scientific Thermo Fisher Scientific Cat# R21001 Hygromycin B Invitrogen Thermo Fisher Scientific Cat# 10687010 Tetracycline hydrochloride Merck Cat# T7660-5G Pierce NeutrAvidinTM Agarose Beads Thermo Scientific Thermo Fisher Scientific Cat# 29201 Hydrogen peroxide solution 30% (w/w) in H2O, contains stabilizer Merck Cat# H1009-5ML Pierce Quantitative assay Thermo Scientific Thermo Fisher Scientific Cat# 23275 TMT10plex Isobaric Label Reagent Set Thermo Scientific Thermo Fisher Scientific Cat# 90110 Sequencing Grade Modified Trypsin, Mass Spec Grade Promega Promega Cat# V5111 Lys-C, Mass Spec Grade Promega Promega Cat# VA1170 Pierce High pH Reversed-Phased Kit Thermo Scientific Thermo Fisher Scientific Cat# 84868 Biotinyl Tyramide (Biotin Phenol) Tocris Bioscience Bio-Techne Cat# 6241 Pierce High Sensitivity NeutrAvidin-HRP Conjugate Thermo Fisher Scientific Thermo Fisher Scientific Cat# 31030 Luminata Western HRP Chemiluminescence Substrate Merck Scientific Laboratory Supplies Cat# WBLUC0100 GloSensor cAMP Reagent stock solution Promega Promega Cat# E1290 DharmaFECT 1 Transfection reagent Dharmacon Reagents Horizon Discovery Cat# T-2001-01 Critical commercial assays LANCE Ultra cAMP assay Perkin Elmer Revvity Cat# TRF0263 HTRF-based cAMP Gαs dynamic kit Revvity Revvity Cat# 62a.m.4PEC Deposited data Source data for mass spectrometry analyses This study PRIDE: PXD053246 Experimental models: Cell lines Flp-In T-REx 293 Francis Crick Institute Cell Facility, UK RRID:CVCL_U427 FLAG-LHR WT HEK293 Hanyaloglu lab; JeanAlphonse et al.22 N/A Flp-In T-REx 293 LHR-APEX2 cells This study N/A (Continued on next page) e1 Cell Chemical Biology 32, 722–735.e1–e7, May 15, 2025 ..

    Article Title: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich Cat#: F1804-1MG; RRID:AB_262044 His-Tag (27E8) Mouse mAb Cell Signaling Cat#: 2366; RRID:AB_2115719 Anti-NanoLuc Monoclonal Antibody Promega Cat#: N7000; RRID: AB_3095534 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody VWR Cat#: 926-32210; RRID:AB_621842 LC3B (D11) XP Rabbit mAb Cell Signaling Cat#: 3868; RRID: AB_2137707 Vinculin (E1E9V) XP Cell Signaling Cat#: 13901; RRID: AB_2728768 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#: C2987H Chemicals, peptides, and recombinant proteins Tetramethylrhodamine (TAMRA)-PEG3-azide Synthesized in-house N/A Biotin-PEG4-azide BroadPharm Cat#: BP-22119 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#: 32106 Novex 4-20% Tris-Glycine Mini Gels Invitrogen Cat#: XP04205BOX Nitrocellulose western blotting membrane, 0.45 mM GE Healthcare Amersham Cat#: 10600002 DMSO Corning Cat#: 25-950-CQC Urea Fisher Scientific Cat#: M1084871000 Iodoacetamide Sigma-Aldrich Cat#: I1149-25G Tris(benzyltriazolylmethyl)amine (TBTA) TCI Cat#: T2993 Copper(II) sulfate, anhydrous Sigma-Aldrich Cat#: 451657-10G Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#: 75259 Sequencing grade modified trypsin Promega Cat#: V5111 Streptavidin agarose resin Fisher Scientific Cat#: 20349 Micro bio-spin column Bio-rad Cat#: 7326204 Triethylammonium bicarbonate (TEAB) buffer Sigma-Aldrich Cat#: T7408-500ML TMT10plex Isobaric Label Reagent Set Thermo Scientific Cat#: 90406 TMT16plex Isobaric Label Reagent Set Thermo Scientific Cat#: 467804-10ML Formic acid, 98%, for mass spectrometry Honeywell Fluka Cat#: 94318-250ML-F Lipofectamine 3000 Transfection Reagent Thermo Scientific Cat#: L3000001 Polybrene Sigma Aldrich Cat#: TR-1003-G puromycin InvivoGen Cat#: ant-pr-1 PI-103 Sigma Aldrich Cat#: 528101 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#: G7573 NanoBRET Nano-Glo Substrate Promega Cat#: N1571 (Continued on next page) Cell Chemical Biology 32, 1–18.e1–e32, March 20, 2025 e1 ..

    Western Blot:

    Article Title: Spatiotemporally resolved GPCR interactome uncovers unique mediators of receptor agonism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-FLAG M1 primary Sigma-Aldrich Cat# F3040, RRID: AB_439712 Goat anti-mouse Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11001, RRID: AB_2534069 Rabbit anti-EEA1 primary Invitrogen Thermo Fisher Scientific Cat# MA5-14794, RRID: AB_10985824 Goat anti-rabbit Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11008, RRID: AB_143165 Goat anti-mouse Alexa Fluor 594-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11005, RRID: AB_2534073 Chemicals, peptides, and recombinant proteins Luteinizing hormone (LH) GoldenWest BioSolutions, LLC Cat# TLIH-A1165.02 Lipofectamine 2000 Transfection Reagent Invitrogen Thermo Fisher Scientific Cat# 12566014 Zeocin Invitrogen Thermo Fisher Scientific Cat# R25001 Blasticidin Thermo Scientific Thermo Fisher Scientific Cat# R21001 Hygromycin B Invitrogen Thermo Fisher Scientific Cat# 10687010 Tetracycline hydrochloride Merck Cat# T7660-5G Pierce NeutrAvidinTM Agarose Beads Thermo Scientific Thermo Fisher Scientific Cat# 29201 Hydrogen peroxide solution 30% (w/w) in H2O, contains stabilizer Merck Cat# H1009-5ML Pierce Quantitative assay Thermo Scientific Thermo Fisher Scientific Cat# 23275 TMT10plex Isobaric Label Reagent Set Thermo Scientific Thermo Fisher Scientific Cat# 90110 Sequencing Grade Modified Trypsin, Mass Spec Grade Promega Promega Cat# V5111 Lys-C, Mass Spec Grade Promega Promega Cat# VA1170 Pierce High pH Reversed-Phased Kit Thermo Scientific Thermo Fisher Scientific Cat# 84868 Biotinyl Tyramide (Biotin Phenol) Tocris Bioscience Bio-Techne Cat# 6241 Pierce High Sensitivity NeutrAvidin-HRP Conjugate Thermo Fisher Scientific Thermo Fisher Scientific Cat# 31030 Luminata Western HRP Chemiluminescence Substrate Merck Scientific Laboratory Supplies Cat# WBLUC0100 GloSensor cAMP Reagent stock solution Promega Promega Cat# E1290 DharmaFECT 1 Transfection reagent Dharmacon Reagents Horizon Discovery Cat# T-2001-01 Critical commercial assays LANCE Ultra cAMP assay Perkin Elmer Revvity Cat# TRF0263 HTRF-based cAMP Gαs dynamic kit Revvity Revvity Cat# 62a.m.4PEC Deposited data Source data for mass spectrometry analyses This study PRIDE: PXD053246 Experimental models: Cell lines Flp-In T-REx 293 Francis Crick Institute Cell Facility, UK RRID:CVCL_U427 FLAG-LHR WT HEK293 Hanyaloglu lab; JeanAlphonse et al.22 N/A Flp-In T-REx 293 LHR-APEX2 cells This study N/A (Continued on next page) e1 Cell Chemical Biology 32, 722–735.e1–e7, May 15, 2025 ..

    Article Title: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich Cat#: F1804-1MG; RRID:AB_262044 His-Tag (27E8) Mouse mAb Cell Signaling Cat#: 2366; RRID:AB_2115719 Anti-NanoLuc Monoclonal Antibody Promega Cat#: N7000; RRID: AB_3095534 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody VWR Cat#: 926-32210; RRID:AB_621842 LC3B (D11) XP Rabbit mAb Cell Signaling Cat#: 3868; RRID: AB_2137707 Vinculin (E1E9V) XP Cell Signaling Cat#: 13901; RRID: AB_2728768 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#: C2987H Chemicals, peptides, and recombinant proteins Tetramethylrhodamine (TAMRA)-PEG3-azide Synthesized in-house N/A Biotin-PEG4-azide BroadPharm Cat#: BP-22119 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#: 32106 Novex 4-20% Tris-Glycine Mini Gels Invitrogen Cat#: XP04205BOX Nitrocellulose western blotting membrane, 0.45 mM GE Healthcare Amersham Cat#: 10600002 DMSO Corning Cat#: 25-950-CQC Urea Fisher Scientific Cat#: M1084871000 Iodoacetamide Sigma-Aldrich Cat#: I1149-25G Tris(benzyltriazolylmethyl)amine (TBTA) TCI Cat#: T2993 Copper(II) sulfate, anhydrous Sigma-Aldrich Cat#: 451657-10G Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#: 75259 Sequencing grade modified trypsin Promega Cat#: V5111 Streptavidin agarose resin Fisher Scientific Cat#: 20349 Micro bio-spin column Bio-rad Cat#: 7326204 Triethylammonium bicarbonate (TEAB) buffer Sigma-Aldrich Cat#: T7408-500ML TMT10plex Isobaric Label Reagent Set Thermo Scientific Cat#: 90406 TMT16plex Isobaric Label Reagent Set Thermo Scientific Cat#: 467804-10ML Formic acid, 98%, for mass spectrometry Honeywell Fluka Cat#: 94318-250ML-F Lipofectamine 3000 Transfection Reagent Thermo Scientific Cat#: L3000001 Polybrene Sigma Aldrich Cat#: TR-1003-G puromycin InvivoGen Cat#: ant-pr-1 PI-103 Sigma Aldrich Cat#: 528101 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#: G7573 NanoBRET Nano-Glo Substrate Promega Cat#: N1571 (Continued on next page) Cell Chemical Biology 32, 1–18.e1–e32, March 20, 2025 e1 ..

    cAMP Assay:

    Article Title: Spatiotemporally resolved GPCR interactome uncovers unique mediators of receptor agonism.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-FLAG M1 primary Sigma-Aldrich Cat# F3040, RRID: AB_439712 Goat anti-mouse Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11001, RRID: AB_2534069 Rabbit anti-EEA1 primary Invitrogen Thermo Fisher Scientific Cat# MA5-14794, RRID: AB_10985824 Goat anti-rabbit Alexa Fluor 488-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11008, RRID: AB_143165 Goat anti-mouse Alexa Fluor 594-conjugated secondary Invitrogen Thermo Fisher Scientific Cat# A-11005, RRID: AB_2534073 Chemicals, peptides, and recombinant proteins Luteinizing hormone (LH) GoldenWest BioSolutions, LLC Cat# TLIH-A1165.02 Lipofectamine 2000 Transfection Reagent Invitrogen Thermo Fisher Scientific Cat# 12566014 Zeocin Invitrogen Thermo Fisher Scientific Cat# R25001 Blasticidin Thermo Scientific Thermo Fisher Scientific Cat# R21001 Hygromycin B Invitrogen Thermo Fisher Scientific Cat# 10687010 Tetracycline hydrochloride Merck Cat# T7660-5G Pierce NeutrAvidinTM Agarose Beads Thermo Scientific Thermo Fisher Scientific Cat# 29201 Hydrogen peroxide solution 30% (w/w) in H2O, contains stabilizer Merck Cat# H1009-5ML Pierce Quantitative assay Thermo Scientific Thermo Fisher Scientific Cat# 23275 TMT10plex Isobaric Label Reagent Set Thermo Scientific Thermo Fisher Scientific Cat# 90110 Sequencing Grade Modified Trypsin, Mass Spec Grade Promega Promega Cat# V5111 Lys-C, Mass Spec Grade Promega Promega Cat# VA1170 Pierce High pH Reversed-Phased Kit Thermo Scientific Thermo Fisher Scientific Cat# 84868 Biotinyl Tyramide (Biotin Phenol) Tocris Bioscience Bio-Techne Cat# 6241 Pierce High Sensitivity NeutrAvidin-HRP Conjugate Thermo Fisher Scientific Thermo Fisher Scientific Cat# 31030 Luminata Western HRP Chemiluminescence Substrate Merck Scientific Laboratory Supplies Cat# WBLUC0100 GloSensor cAMP Reagent stock solution Promega Promega Cat# E1290 DharmaFECT 1 Transfection reagent Dharmacon Reagents Horizon Discovery Cat# T-2001-01 Critical commercial assays LANCE Ultra cAMP assay Perkin Elmer Revvity Cat# TRF0263 HTRF-based cAMP Gαs dynamic kit Revvity Revvity Cat# 62a.m.4PEC Deposited data Source data for mass spectrometry analyses This study PRIDE: PXD053246 Experimental models: Cell lines Flp-In T-REx 293 Francis Crick Institute Cell Facility, UK RRID:CVCL_U427 FLAG-LHR WT HEK293 Hanyaloglu lab; JeanAlphonse et al.22 N/A Flp-In T-REx 293 LHR-APEX2 cells This study N/A (Continued on next page) e1 Cell Chemical Biology 32, 722–735.e1–e7, May 15, 2025 ..

    Labeling:

    Article Title: Autophosphorylation of the Tousled-like kinases TLK1 and TLK2 regulates recruitment to damaged chromatin via PCNA interaction.
    Article Snippet: .. One hundred twenty microgram of peptides were labeled using a TMT10plex isobaric label reagent set with the addition of the TMT11-131C label (Thermo, A34808). .. Labeled peptides were dried by speed-vac and resuspended in phosphopeptides binding / wash buffer from the High-Select TiO 2 phosphopeptide enrichment kit (Pierce, A32993).

    Produced:

    Article Title: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich Cat#: F1804-1MG; RRID:AB_262044 His-Tag (27E8) Mouse mAb Cell Signaling Cat#: 2366; RRID:AB_2115719 Anti-NanoLuc Monoclonal Antibody Promega Cat#: N7000; RRID: AB_3095534 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody VWR Cat#: 926-32210; RRID:AB_621842 LC3B (D11) XP Rabbit mAb Cell Signaling Cat#: 3868; RRID: AB_2137707 Vinculin (E1E9V) XP Cell Signaling Cat#: 13901; RRID: AB_2728768 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#: C2987H Chemicals, peptides, and recombinant proteins Tetramethylrhodamine (TAMRA)-PEG3-azide Synthesized in-house N/A Biotin-PEG4-azide BroadPharm Cat#: BP-22119 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#: 32106 Novex 4-20% Tris-Glycine Mini Gels Invitrogen Cat#: XP04205BOX Nitrocellulose western blotting membrane, 0.45 mM GE Healthcare Amersham Cat#: 10600002 DMSO Corning Cat#: 25-950-CQC Urea Fisher Scientific Cat#: M1084871000 Iodoacetamide Sigma-Aldrich Cat#: I1149-25G Tris(benzyltriazolylmethyl)amine (TBTA) TCI Cat#: T2993 Copper(II) sulfate, anhydrous Sigma-Aldrich Cat#: 451657-10G Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#: 75259 Sequencing grade modified trypsin Promega Cat#: V5111 Streptavidin agarose resin Fisher Scientific Cat#: 20349 Micro bio-spin column Bio-rad Cat#: 7326204 Triethylammonium bicarbonate (TEAB) buffer Sigma-Aldrich Cat#: T7408-500ML TMT10plex Isobaric Label Reagent Set Thermo Scientific Cat#: 90406 TMT16plex Isobaric Label Reagent Set Thermo Scientific Cat#: 467804-10ML Formic acid, 98%, for mass spectrometry Honeywell Fluka Cat#: 94318-250ML-F Lipofectamine 3000 Transfection Reagent Thermo Scientific Cat#: L3000001 Polybrene Sigma Aldrich Cat#: TR-1003-G puromycin InvivoGen Cat#: ant-pr-1 PI-103 Sigma Aldrich Cat#: 528101 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#: G7573 NanoBRET Nano-Glo Substrate Promega Cat#: N1571 (Continued on next page) Cell Chemical Biology 32, 1–18.e1–e32, March 20, 2025 e1 ..

    Virus:

    Article Title: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich Cat#: F1804-1MG; RRID:AB_262044 His-Tag (27E8) Mouse mAb Cell Signaling Cat#: 2366; RRID:AB_2115719 Anti-NanoLuc Monoclonal Antibody Promega Cat#: N7000; RRID: AB_3095534 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody VWR Cat#: 926-32210; RRID:AB_621842 LC3B (D11) XP Rabbit mAb Cell Signaling Cat#: 3868; RRID: AB_2137707 Vinculin (E1E9V) XP Cell Signaling Cat#: 13901; RRID: AB_2728768 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#: C2987H Chemicals, peptides, and recombinant proteins Tetramethylrhodamine (TAMRA)-PEG3-azide Synthesized in-house N/A Biotin-PEG4-azide BroadPharm Cat#: BP-22119 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#: 32106 Novex 4-20% Tris-Glycine Mini Gels Invitrogen Cat#: XP04205BOX Nitrocellulose western blotting membrane, 0.45 mM GE Healthcare Amersham Cat#: 10600002 DMSO Corning Cat#: 25-950-CQC Urea Fisher Scientific Cat#: M1084871000 Iodoacetamide Sigma-Aldrich Cat#: I1149-25G Tris(benzyltriazolylmethyl)amine (TBTA) TCI Cat#: T2993 Copper(II) sulfate, anhydrous Sigma-Aldrich Cat#: 451657-10G Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#: 75259 Sequencing grade modified trypsin Promega Cat#: V5111 Streptavidin agarose resin Fisher Scientific Cat#: 20349 Micro bio-spin column Bio-rad Cat#: 7326204 Triethylammonium bicarbonate (TEAB) buffer Sigma-Aldrich Cat#: T7408-500ML TMT10plex Isobaric Label Reagent Set Thermo Scientific Cat#: 90406 TMT16plex Isobaric Label Reagent Set Thermo Scientific Cat#: 467804-10ML Formic acid, 98%, for mass spectrometry Honeywell Fluka Cat#: 94318-250ML-F Lipofectamine 3000 Transfection Reagent Thermo Scientific Cat#: L3000001 Polybrene Sigma Aldrich Cat#: TR-1003-G puromycin InvivoGen Cat#: ant-pr-1 PI-103 Sigma Aldrich Cat#: 528101 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#: G7573 NanoBRET Nano-Glo Substrate Promega Cat#: N1571 (Continued on next page) Cell Chemical Biology 32, 1–18.e1–e32, March 20, 2025 e1 ..

    Synthesized:

    Article Title: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich Cat#: F1804-1MG; RRID:AB_262044 His-Tag (27E8) Mouse mAb Cell Signaling Cat#: 2366; RRID:AB_2115719 Anti-NanoLuc Monoclonal Antibody Promega Cat#: N7000; RRID: AB_3095534 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody VWR Cat#: 926-32210; RRID:AB_621842 LC3B (D11) XP Rabbit mAb Cell Signaling Cat#: 3868; RRID: AB_2137707 Vinculin (E1E9V) XP Cell Signaling Cat#: 13901; RRID: AB_2728768 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#: C2987H Chemicals, peptides, and recombinant proteins Tetramethylrhodamine (TAMRA)-PEG3-azide Synthesized in-house N/A Biotin-PEG4-azide BroadPharm Cat#: BP-22119 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#: 32106 Novex 4-20% Tris-Glycine Mini Gels Invitrogen Cat#: XP04205BOX Nitrocellulose western blotting membrane, 0.45 mM GE Healthcare Amersham Cat#: 10600002 DMSO Corning Cat#: 25-950-CQC Urea Fisher Scientific Cat#: M1084871000 Iodoacetamide Sigma-Aldrich Cat#: I1149-25G Tris(benzyltriazolylmethyl)amine (TBTA) TCI Cat#: T2993 Copper(II) sulfate, anhydrous Sigma-Aldrich Cat#: 451657-10G Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#: 75259 Sequencing grade modified trypsin Promega Cat#: V5111 Streptavidin agarose resin Fisher Scientific Cat#: 20349 Micro bio-spin column Bio-rad Cat#: 7326204 Triethylammonium bicarbonate (TEAB) buffer Sigma-Aldrich Cat#: T7408-500ML TMT10plex Isobaric Label Reagent Set Thermo Scientific Cat#: 90406 TMT16plex Isobaric Label Reagent Set Thermo Scientific Cat#: 467804-10ML Formic acid, 98%, for mass spectrometry Honeywell Fluka Cat#: 94318-250ML-F Lipofectamine 3000 Transfection Reagent Thermo Scientific Cat#: L3000001 Polybrene Sigma Aldrich Cat#: TR-1003-G puromycin InvivoGen Cat#: ant-pr-1 PI-103 Sigma Aldrich Cat#: 528101 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#: G7573 NanoBRET Nano-Glo Substrate Promega Cat#: N1571 (Continued on next page) Cell Chemical Biology 32, 1–18.e1–e32, March 20, 2025 e1 ..

    Membrane:

    Article Title: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich Cat#: F1804-1MG; RRID:AB_262044 His-Tag (27E8) Mouse mAb Cell Signaling Cat#: 2366; RRID:AB_2115719 Anti-NanoLuc Monoclonal Antibody Promega Cat#: N7000; RRID: AB_3095534 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody VWR Cat#: 926-32210; RRID:AB_621842 LC3B (D11) XP Rabbit mAb Cell Signaling Cat#: 3868; RRID: AB_2137707 Vinculin (E1E9V) XP Cell Signaling Cat#: 13901; RRID: AB_2728768 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#: C2987H Chemicals, peptides, and recombinant proteins Tetramethylrhodamine (TAMRA)-PEG3-azide Synthesized in-house N/A Biotin-PEG4-azide BroadPharm Cat#: BP-22119 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#: 32106 Novex 4-20% Tris-Glycine Mini Gels Invitrogen Cat#: XP04205BOX Nitrocellulose western blotting membrane, 0.45 mM GE Healthcare Amersham Cat#: 10600002 DMSO Corning Cat#: 25-950-CQC Urea Fisher Scientific Cat#: M1084871000 Iodoacetamide Sigma-Aldrich Cat#: I1149-25G Tris(benzyltriazolylmethyl)amine (TBTA) TCI Cat#: T2993 Copper(II) sulfate, anhydrous Sigma-Aldrich Cat#: 451657-10G Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#: 75259 Sequencing grade modified trypsin Promega Cat#: V5111 Streptavidin agarose resin Fisher Scientific Cat#: 20349 Micro bio-spin column Bio-rad Cat#: 7326204 Triethylammonium bicarbonate (TEAB) buffer Sigma-Aldrich Cat#: T7408-500ML TMT10plex Isobaric Label Reagent Set Thermo Scientific Cat#: 90406 TMT16plex Isobaric Label Reagent Set Thermo Scientific Cat#: 467804-10ML Formic acid, 98%, for mass spectrometry Honeywell Fluka Cat#: 94318-250ML-F Lipofectamine 3000 Transfection Reagent Thermo Scientific Cat#: L3000001 Polybrene Sigma Aldrich Cat#: TR-1003-G puromycin InvivoGen Cat#: ant-pr-1 PI-103 Sigma Aldrich Cat#: 528101 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#: G7573 NanoBRET Nano-Glo Substrate Promega Cat#: N1571 (Continued on next page) Cell Chemical Biology 32, 1–18.e1–e32, March 20, 2025 e1 ..

    Cell Viability Assay:

    Article Title: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich Cat#: F1804-1MG; RRID:AB_262044 His-Tag (27E8) Mouse mAb Cell Signaling Cat#: 2366; RRID:AB_2115719 Anti-NanoLuc Monoclonal Antibody Promega Cat#: N7000; RRID: AB_3095534 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody VWR Cat#: 926-32210; RRID:AB_621842 LC3B (D11) XP Rabbit mAb Cell Signaling Cat#: 3868; RRID: AB_2137707 Vinculin (E1E9V) XP Cell Signaling Cat#: 13901; RRID: AB_2728768 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#: C2987H Chemicals, peptides, and recombinant proteins Tetramethylrhodamine (TAMRA)-PEG3-azide Synthesized in-house N/A Biotin-PEG4-azide BroadPharm Cat#: BP-22119 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#: 32106 Novex 4-20% Tris-Glycine Mini Gels Invitrogen Cat#: XP04205BOX Nitrocellulose western blotting membrane, 0.45 mM GE Healthcare Amersham Cat#: 10600002 DMSO Corning Cat#: 25-950-CQC Urea Fisher Scientific Cat#: M1084871000 Iodoacetamide Sigma-Aldrich Cat#: I1149-25G Tris(benzyltriazolylmethyl)amine (TBTA) TCI Cat#: T2993 Copper(II) sulfate, anhydrous Sigma-Aldrich Cat#: 451657-10G Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#: 75259 Sequencing grade modified trypsin Promega Cat#: V5111 Streptavidin agarose resin Fisher Scientific Cat#: 20349 Micro bio-spin column Bio-rad Cat#: 7326204 Triethylammonium bicarbonate (TEAB) buffer Sigma-Aldrich Cat#: T7408-500ML TMT10plex Isobaric Label Reagent Set Thermo Scientific Cat#: 90406 TMT16plex Isobaric Label Reagent Set Thermo Scientific Cat#: 467804-10ML Formic acid, 98%, for mass spectrometry Honeywell Fluka Cat#: 94318-250ML-F Lipofectamine 3000 Transfection Reagent Thermo Scientific Cat#: L3000001 Polybrene Sigma Aldrich Cat#: TR-1003-G puromycin InvivoGen Cat#: ant-pr-1 PI-103 Sigma Aldrich Cat#: 528101 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#: G7573 NanoBRET Nano-Glo Substrate Promega Cat#: N1571 (Continued on next page) Cell Chemical Biology 32, 1–18.e1–e32, March 20, 2025 e1 ..



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    Reagents and tools table

    Journal: Molecular Systems Biology

    Article Title: Gemcitabine and ATR inhibitors synergize to kill PDAC cells by blocking DNA damage response

    doi: 10.1038/s44320-025-00085-6

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: TMT10plex Isobaric Label Reagent Set , Thermo Fisher Scientific , 90406.

    Techniques: Labeling, Recombinant, Luminescence Assay, Cell Viability Assay, Software, Mass Spectrometry